Journal: The Journal of antimicrobial chemotherapy

Article Title: Evaluation of the new GenoType NTM-DR kit for the molecular detection of antimicrobial resistance in non-tuberculous mycobacteria.

doi: 10.1093/jac/dkx021

Figure Lengend Snippet: Figure 2. Representative GenoType NTM-DR DNA strip results obtained with NTM isolates showing representative profiles. Lanes 1 and 2, M. abscessus subsp. massiliense with rrl and rrs mutations in lane 1 and WT genes in lane 2; lane 3, M. abscessus subsp. bolletii WT for rrl and rrs; lane 4, M. abscessus subsp. abscessus erm(41) t28 genotype and rrl and rrs mutations (heterogeneous for rrs with a faint WT band); lane 5, M. abscessus subsp. abscessus erm(41) t28 genotype and WT for rrl and rrs; lane 6, M. abscessus subsp. abscessus erm(41) c28 genotype and WT for rrl and rrs; lane 7, M. chelonae with an rrl mutation and WT rrs; lane 8, M. chelonae heterogeneous for rrl (WT and rrl mutation) and WT rrs; lane 9, M. chelonae WT for rrl and rrs; lane 10 M. chimaera with a dou- ble mutation in rrl and WT for rrs; lane 11, M. chimaera WT for rrl and rrs; lane 12, M. intracellulare with an rrl mutation and WT for rrs; lane 13, M. intracellulare WT for rrl and rrs; lane 14, M. avium with a double rrl mutation and WT for rrs; lane 15, M. avium with one rrl mutation and one in rrs (heterogeneous profile with a faint WT band); lane 16, M. avium heterogeneous for rrl (WT and mutation) and WT for rrs; lane 17, M. avium WT for rrl and rrs. CC, conjugate control; UC, universal control.

Article Snippet: DNA extracted from two suspensions of M. abscessus (DNA concentrations of 36.1 and 42.2 ng/lL for strains ATCC 19977 and 5-GA-Mab, respectively) yielded positive results for all expected bands down to a low concentration of 0.4 ng/lL.

Techniques: Stripping Membranes, Mutagenesis, Control

Journal: PLOS One

Article Title: A novel variant in MYBPC3 causes hypertrophic cardiomyopathy by haploinsufficiency

doi: 10.1371/journal.pone.0333096

Figure Lengend Snippet: The studied variant is indicated in the representation. cMyBP-C is composed of 11 domains, numbered C0 to C10 from the N-terminus to C-terminus. It includes 8 immunoglobulin (IgI) domains (circles) and 3 fibronectin type III (FnIII) domains (rectangles). Four specific phosphorylation sites are located between C1 and C2, known as MyBP-C motif. The regions of interaction with other sarcomeric proteins (actin, myosin, and titin) are shown with dotted arrows. The relationship between the 35 exons of MYBPC3 cDNA and cMyBP-C domains is indicated by dashed lines.

Article Snippet: The membranes were then incubated with primary antibodies against MYBPC3 (Cat No.19977-1-AP, Proteintech) and tubulin (Cat No.11224-1-AP, Proteintech) at 4°C overnight.

Techniques: Variant Assay, Phospho-proteomics

Journal: PLOS One

Article Title: A novel variant in MYBPC3 causes hypertrophic cardiomyopathy by haploinsufficiency

doi: 10.1371/journal.pone.0333096

Figure Lengend Snippet: (A) RT-qPCR analysis showing significantly decreased MYBPC3 mRNA levels in cardiac sample from the proband compared to control subjects. (B) Representative Western blot images demonstrating reduced cMyBP-C protein expression in the HCM heart relative to controls. (C) Semi-quantification of cMyBP-C protein levels normalized to tubulin. Data are presented as mean ± standard deviation (SD); * P < 0.05 versus controls. HCM = hypertrophic cardiomyopathy.

Article Snippet: The membranes were then incubated with primary antibodies against MYBPC3 (Cat No.19977-1-AP, Proteintech) and tubulin (Cat No.11224-1-AP, Proteintech) at 4°C overnight.

Techniques: Quantitative RT-PCR, Control, Western Blot, Expressing, Standard Deviation